A student performed the following steps in a Southern blot experiment to determine the number of cop

Question

A student performed the following steps in a Southern blot experiment to determine the number of copies of a particular gene that has been inserted in a genetically modified organism.\ (i) Transfer of DNA to nitrocellulose membrane.\ (ii) Restriction digestion of genomic DNA.\ (iii) Cleaved DNA separated using gel electrophoresis.\ (iv) Create radioactive probe.\ (v) Incubate probe and membrane.\ Which is the correct sequence to the above steps?\

Professor PC Thomas Classes · Answer

(a)\