Identify the desirable characteristics for a plasmid used in rDNA technology from the following.\ A

Question

Identify the desirable characteristics for a plasmid used in rDNA technology from the following.\ A. Ability to multiply and express outside the host in a bioreactor.\ B. A highly active promoter.\ C. A site at which replication can be initiated.\ D. Identifiable selectable marker genes.\ E. One or more unique restriction sites.\

Professor PC Thomas Classes · Answer

(c) : Plasmids are extrachromosomal DNA present naturally in a bacterial cell. They are small double-stranded and closed circular DNA. They are used in recombinant DNA technology as cloning and expression vectors. For acting as a vehicle for the desired gene, they should be able to multiply inside the host cell thus need a replication origin site, should have marker genes and unique restriction sites.